Human mesenchymal stromal cells have extensive potential in research: uses for these including tissue repair and tissue engineering, gene therapy, the study of mesenchymal tumor oncogenes, and the analysis of cell fate determination and differentiation. The iMSC#3 cells express markers found in both primary human mesenchymal stromal cells and the multipotent form: CD90, CD44, NT5E, THY1, ENG, alanyl aminopeptidase (ANPEP), integrin beta I (ITGB1), integrin alpha 5 (ITGA5), major histocompatibililty complex, class I, A and B (HLA-A and B), and activated leukocyte cell adhesion molecule (ALCAM). These cells have also shown the ability to differentiate into adipocytes and osteoblasts. Furthermore, the cells have been verified for normal copy number, and lacks chromosomal aberrations and tumorigenicity.
The immortalized nontumorigenic iMSC#3 cell line maintains the main characteristics found in primary hMSCs, thus making this a valuable model for studying basic mesenchymal biology and differentiation, and functional studies of candidate genes involved in mesenchymal tumors.
Naderi, EH et al. "Bone marrow stroma-derived PGE2 protects BCP-ALL cells from DNA damage-induced p53 accumulation and cell death." Mol Cancer 14(1):14 (2015). PubMed: PMC4323193.
Skårn, M et al. "Generation and characterization of an immortalized human mesenchymal stromal cell line." Stem Cells Dev 23(19):2377-89 (2014). PubMed: 24857590 .
