Monoclonal Mouse
Anti-Human CD69, Clone FN50
CD69 is a phosphorylated disulphide-linked dimer composed of two chains of 26 to 28 kDa and 32 to 34 kDa, also known as Activation Inducer Molecule (AIM). CD69 is the earliest known surface antigen expressed on lymphocytes after T or B cell activation but absent from resting lymphocytes. Other cells, including activated macrophages, natural killer (NK) cells, eosinophils, neutrophils and platelets may also express CD69. In vitro studies have demonstrated a transient expression of CD69 on activated T cells. After activation, surface expression can be detected within 2-4 hours, reaching a maximum after 18-24 hours followed by a rapid decrease. CD69 is thus detectable prior to other activation antigens like CD25 and CD71. CD69 is believed to be involved in signal transduction, since cross-linking with anti-CD69 antibodies induce activation.In combination with anti-CD4, anti-CD8, and anti-CD3 antibodies, anti-CD69 may be useful for T cell activation studies.
References:
Beiske K, AAS-Eng DA, Smeland EB, Sundan A, Marton PF, Funderud S. A4.2 Immunohistological and functional characterization of the mAb A91 (FN 50)-reactive activation antigen (CD69) expressed on subsets of normal and neoplastic lymphoid cells. In Knapp W et al., eds. Leucocyte Typing IV. White Cell Differentiation Antigens. Oxford-New York-Tokyo: Oxford University Press, 1989:436-9.
Cebrián M, Sánchez-Mateos P, Redondo JM, Ursa A, De Landázuri MO, Sánchez-Madrid F. A4.4 CD69:a GP33/27 kDa activation inducer molecule (AIM) recognized by a group of mAb of the workshop activation panel. Induction of T-cell proliferation through the AIM activation antigen. In Knapp W et al., eds. Leucocyte Typing IV. White Cell Differentiation Antigens. Oxford-New York-Tokyo: Oxford University Press, 1989:441-4.
Hamann J, Fiebig H, Strauss M. AA4.1 Expression cloning and chromosomal sublocalization of the early activation antigen CD69. In: Schlossman SF et al., eds. Leucocyte Typing V. White Cell Differentiation Antigens. Oxford-New York-Tokyo: Oxford University Press, 1995:1125-6.
