Species: Homo sapiens
Source: Escherichia coli
Description: E.coli expressed, recombinant human glutaryl-CoA dehydrogenase (GCDH), mitochondrial. Produced with N-terminal 6xHis-tag and corresponds to amino acids: 45-438 of human GCDH (NP_000150.1).
Function: GCDH catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism and utilizes electron transfer flavoprotein as its electron acceptor.
Amino Acid sequence: MGSSHHHHHHSSGLVPRGSHMRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITGIQAFTASK
Formulation: intensely yellow liquid, containing 6xHis-tag human GCDH, stored in 20 mM Tris-HCl, pH 8.0, 10% glycerol, 1 µM FAD.
Purity: greater than 95% as determined by SDS-PAGE and Coomassie staining.
Concentration: greater than 5 mg/ml, as determined by BCA test.
Storage: Below minus 20°C. Minimize the number of freeze-thaw cycles.
Activity: Oxidation of glutaryl-CoA. Reduction of ETF (electron transfer flavoprotein)
References: Małecki, J., Ho, A. Y., Moen, A., Dahl, H. A., and Falnes, P. Ø. (2015) Human METTL20 is a mitochondrial lysine methyltransferase that targets the b subunit of electron transfer flavoprotein (ETFb) and modulates its activity. J. Biol. Chem. 290, 423–434.