TET protein is plays an active role in active DNA demethylation.. The Ten-Eleven-Translocation (TET) proteins TET1, TET2, and TET3 are α-ketoglutarate and Fe2+-dependent enzymes capable of modifying DNA methylation status by converting 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC)
Thera re two proteins TET and thymine DNA glycosylase (TDG) initiate active DNA demethylation by oxidation and excision of 5mC in DNA and the anticipated downstream events will be the excision and repair of the resulting abasic site (AP-site) by the DNA base excision repair (BER) system.
To examine the activity of the TET–TDG complex, the catalytic domain of TET1 (His6–TET1CD) and TDG–GST was co-expressed in E. coli and enriched the complexes by Ni-NTA chromatography.
