Recombinant protein purified from E. coli, consisting of GST fused to the Cdc42 (p21) binding domain (CRIB) of the human p21 activated kinase 1 protein (PAK).
GST-PAK-CRIB binds specifically to GTP-bound (but not GDP-bound) Cdc42 small GTPase. It can be used to specifically precipitate active, GTP-bound Cdc42 from cell lysates.
GST-PAK-CRIB contains residues 67-150 of PAK. This region includes the highly conserved CRIB motif plus sequences required for the high affinity interaction with GTP-Cdc42.
The protein is shipped in a glutathione agarose-bound form (non-covalently bound, 10% slurry, 50% glycerol).
Recommended use: 100 µl of PAK-CRIB beads per sample.