Mouse anti-human C9 neoepitope antibody.
Excellent antibody to measure soluble terminal complement complex (TCC) (sC5b-9) as capture antibody in ELISA and to detect deposition of the membrane from of TCC (C5b-9=membrane attack complex (MAC) ) in frozen tissue sections.
The TCC consisting of C5b, C6, C7, C8, and C9, contains neoantigens that are absent from the individual native components. Neoantigens are present both in the MAC and the fluid-phase (SC5b-9) complex. This membrane attack complex forms channels in target cell membranes which is leading by osmotic leakag to cell lysis. The complexes contain neoantigens that are absent from the individual native components from which they are formed and DIA 011-01 is directed against a neoepitope exposed on C9 when incorporated into the TCC.
Clone number: aE11
Epitope: DIA 011-01 binds to a neoepitope exposed on C9
Specificity: DIA 011-01 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive)
Link to publications:
1.Mollnes TE, Harboe M (1987) Immunohistochemical detection of the membrane and fluid-phase terminal complement complexes C5b-9(m) and SC5b-9. Consequences for interpretation and terminology. Scand J Immunol 26:381-6
2. Mollnes TE, Lea T, Harboe M, Tschopp J (1985) Monoclonal antibodies recognizing a neoantigen of poly (C9) detect the human terminal complement complex in tissue and plasma. Scand J Immunol 22:183-1
3. Mollnes TE (1997) Analysis of in vivo complement activation. Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C: Weir's Handbook of Experimental Immunology. Boston, MA: Blackwell Science, pp. 78.1-78.8.